Novel Identification Technique of Gold Nanoparticles Biosynthesized by "Mesobacillus Foraminis ShAm 98 Iraq" Bacteria Isolated from Hot Spring Water at Hammam A-Aleel in Mosul/ Iraq

A new strain Mesobacillus foraminis (M. foraminis) locally isolated from hot spring water at Hammam Al-Aleel in the south of Mosul city/ Iraq was identified using phylogenetic analysis. Its ability to synthesize gold nanoparticles has been studied for the first time as a micro-factory for GNPs by observing the colour change. To prove the formation of GNPs, X-diffraction (XRD) analysis was done and the best formation of crystalline GNPs was observed at the (III) crystal plane. Atomic Force Microscope (AFM) technique was used to determine the shape, size, and surface smoothness of nanoparticles, while Transmission and Scanning Electron Microscope (TEM and SEM, respectively) were applied to characterize the size and morphology. Moreover, fluorescent microscope was used for the first time as a novel method in determining the characteristic morphology of GNPs polymers by using acridine orange which used for GNPs staining and examined under a fluorescent microscope. The fluorescent images show amazing GNPs polymers with shiny spots with more details of dendroid structures in the polymers. Since there was no previous study on using a fluorescent microscope and acridine orange stain in the field of nanotechnology, this study is considered the first effort to characterize biosynthesized GNPs using this technique.