Investigation of some Carboxylic Acids and Phenolic Compounds of Ailanthus altissima Leaves and their its Effect on Italian Cupressus Seedlings Root Rot Fungi

The study was carried out for separation and identification of some carboxylic acids such as Aspartic, Citric, Tartaric, Propionic, Ascorbic, Maleic and Fumaric, Adepic acid, as well as Phenol, Resorcinol, Hydroguinone, Quercetine, p-Hydroxybenzoic, Benzoic acid and Gallic acid from Alianthus altissima determined by High Performance Liquid Chromatography (HPLC). Moreover the major components of carboxylic acid were presented as Ascorbic acid (86.38%). Also the phenolic compounds was studied and the results showed that P- Hydroxy benzoic acid (41.99%) was the highest amount. Isolation results from Cupressus seedlings infected with root rot disease appearance of the fungus F. solani, F. oxysporum , F. chlamydosporium and Rhizoctonia solani, the isolation ratios was 41% as amaximum value for F. solani, then F. oxyspoum (27%), F. chlamydosporium (12%) which was minimum value and Rhizoctonia solani (20%). Bioassay results of Ailanthus altissima leaves extract showed an increasing inhibition ratio of fungi growth with increasing leaves extract concentrations, F.oxysporum and F.chlamydosporium had the highest degree of growth inhibition (100) % for the two fungus at (4%) extracts concentrations, then followed by the fungus F.solani and Rhizoctonia solani were (84. 78,57) % respectively and Rhizoctonia solani had the minimum inhibition. The results of average fungus growth treated with several concentrations of Ailanthus leaves extracts with PDA media showed inhibition of growth with increasing extracts concentration, F. chlamydospoum had minimum mean growth 7mm at 1% extract conc. whereas the two species of Fusarium showed maximum inhibition for average growth were zero values at 4% of extract conc. except Rhizoctonia solani which had a different value (19.67) mm at the same conc. . 86.38 % .% 41.99 F. Fusarium solani 41 % Rhizoctonia solani F. chlamydosporium oxysporum F. chlamydosporium 27 % F. oxysporum F. solani 20 % Rhizoctonia solani ( 12 )% F. Ailanthus altissima F. solani 100 % 4% chlamydosporium Rhizoctonia ( 84 57 87 )% Rhizoctonia solani . solani 7 F. chlamydosporium Fusarium 1% 19.67