Impact of Phytogenic Feed Additives on Growth Performance, Antioxidant Defense, and Serum Biochemical Indices in Cirrhinus mrigala

Section: Article
Issue
Vol. 34 No. 4 (2025): Volume 34 Issue 4
Published
Oct 1, 2025
Pages
86-96

Abstract

This study investigated the effects of dietary supplementation with Moringa oleifera, Allium sativum, Zingiber officinale, and Curcuma longa powders (each at 2% inclusion) on Cirrhinus mrigala over a 60-day feeding trial on growth, antioxidant status, and serum biochemistry. A total of 270 juvenile fish (15.8 ± 0.12 g) were distributed into five groups (control and four treatments) in triplicate. Growth performance, antioxidant enzyme activities (Superoxide dismutase; SOD, Catalase; CAT, and Malondialdehyde; MDA), serum biochemical parameters (Total protein, albumin, globulin), and hepatic enzyme activities (Alanine aminotransferase; ALT, and Aspartate aminotransferase; AST) were measured. Turmeric (T4) and garlic (T2) significantly improved growth performance, with T4 exhibiting the highest weight gain and best feed conversion ratio. Both T4 and T2 markedly increased SOD and CAT activities while reducing MDA levels. Serum analysis revealed improved hepatic health. These results demonstrate the potential of turmeric and garlic as sustainable phytogenic additives for improving productivity, oxidative resilience, and health in C. mrigala aquaculture, providing viable alternatives to synthetic growth promoters.

References

  1. J. A. Gephart, C. D. Golden, F. Asche, B. Belton, C. Brugere, H. E. Froehlich, and E. H. Allison, “Scenarios for global aquaculture and its role in human nutrition,” Rev. Fish. Sci. Aquac., vol. 29, no. 1, pp. 122–138, 2020. http://dx.doi.org/10.1080/23308249.2020.1782342
  2. G. S. Araujo, J. W. A. D. Silva, J. Cotas, and L. Pereira, “Fish farming techniques: current situation and trends,” J. Mar. Sci. Eng., vol. 10, no. 11, p. 1598, 2022. http://dx.doi.org/10.3390/jmse10111598
  3. S. J. Kargbo, N. Barzkar, D. Y. Cole, K. D. Kamara, A. Soratur, R. Othman, ... and B. A. V. Maran, “A comprehensive overview of the current fisheries status in Sierra Leone,” Afr. J. Biol. Sci., vol. 6, no. 6, pp. 8028–8056, 2024. http://dx.doi.org/10.33472/AFJBS.6.6.2024.8028-8056
  4. M. Mugwanya, M. A. Dawood, F. Kimera, and H. Sewilam, “A review on recirculating aquaculture system: Influence of stocking density on fish and crustacean behavior, growth performance, and immunity,” Ann. Anim. Sci., vol. 22, p. 873, 2022. http://dx.doi.org/10.2478/aoas-2022-0014
  5. D. Roy, P. K. Saha, S. Sarker, M. A. Parves, A. P. Zavyalov, and G. A. Latifa, “Culture practice of Oreochromis niloticus through recirculating aquaculture system (RAS): a modern and growth-optimized approach of fish culture by maintaining water quality with proper fish stocking density in Bangladesh,” Environ. Sci. Pollut. Res., vol. 32, no. 18, pp. 11749–11766, 2025. http://dx.doi.org/10.1007/s11356-025-36386-4
  6. A. Gupta, S. K. Gupta, M. Priyam, M. A. Siddik, N. Kumar, P. K. Mishra, ... and A. Pattanayak, “Immunomodulation by dietary supplements: A preventive health strategy for sustainable aquaculture of tropical freshwater fish, Labeo rohita (Hamilton, 1822),” Rev. Aquac., vol. 13, no. 4, pp. 2364–2394, 2021. http://dx.doi.org/10.1111/raq.12581
  7. N. K. Yadav, A. B. Patel, S. K. Singh, N. K. Mehta, V. Anand, J. Lal, and N. C. Devi, “Climate change effects on aquaculture production and its sustainable management through climate-resilient adaptation strategies: a review,” Environ. Sci. Pollut. Res., vol. 31, no. 22, pp. 31731–31751, 2024. http://dx.doi.org/10.1007/s11356-024-33397-5
  8. S. Biswas, J. M. Ahn, and I. H. Kim, “Assessing the potential of phytogenic feed additives: A comprehensive review on their effectiveness as a potent dietary enhancement for nonruminant in swine and poultry,” J. Anim. Physiol. Anim. Nutr., vol. 108, no. 3, pp. 711–723, 2024. http://dx.doi.org/10.1111/jpn.13922
  9. M. Madesh, J. Yan, G. Jinan, P. Hu, I. H. Kim, H. Y. Liu, ... and D. Cai, “Phytogenics in swine nutrition and their effects on growth performance, nutrient utilization, gut health, and meat quality: a review,” Stress Biol., vol. 5, no. 1, p. 11, 2025. http://dx.doi.org/10.1007/s44154-024-00209-2
  10. U. E. Obianwuna, X. Chang, V. U. Oleforuh-Okoleh, P. N. Onu, H. Zhang, K. Qiu, and S. Wu, “Phytobiotics in poultry: revolutionizing broiler chicken nutrition with plant-derived gut health enhancers,” J. Anim. Sci. Biotechnol., vol. 15, no. 1, p. 169, 2024. http://dx.doi.org/10.1186/s40104-024-01101-9
  11. F. K. Amevor, V. A. Uyanga, L. Wu, D. Xu, G. Shu, Y. Wang, and X. Zhao, “Enhancing poultry health and productivity through the liver-gut axis with integrated nutritional and immunological approaches: a mini-review,” Front. Physiol., vol. 16, p. 1537099, 2025. http://dx.doi.org/10.3389/fphys.2025.1537099
  12. Z. Sajid, A. K. Gamperl, C. C. Parrish, S. M. Colombo, J. Santander, C. Mather, ... and G. G. Singh, “An aquaculture risk model to understand the causes and consequences of Atlantic Salmon mass mortality events: A review,” Rev. Aquac., vol. 16, no. 4, pp. 1674–1695, 2024. http://dx.doi.org/10.1111/raq.12917
  13. S. L. Sumana, T. Xue, H. Hu, M. M. Abdullateef, Y. Shui, G. U. Ayana, ... and J. Xiaojun, “Medicinal plants as ecological solutions for fish growth and immunostimulatory effects in aquaculture,” Aquac. Res., vol. 2025, no. 1, p. 9778623, 2025. http://dx.doi.org/10.1155/are/9778623
  14. A. Khan, S. Abass, S. A. I. Nizami, M. Shariq, S. Zahiruddin, B. Parveen, and R. Parveen, “The gut health revolution: Herbs and dietary phytochemicals in balancing gut microbiota for optimal human health,” Curr. Pharm. Biotechnol., 2024. http://dx.doi.org/10.1371/journal.pone.0308761
  15. M. T. El-Saadony, A. M. Saad, D. M. Mohammed, S. A. Korma, M. Y. Alshahrani, A. E. Ahmed, and S. A. Ibrahim, “Medicinal plants: bioactive compounds, biological activities, combating multidrug-resistant microorganisms, and human health benefits a comprehensive review,” Front. Immunol., vol. 16, p. 1491777, 2025. http://dx.doi.org/10.3389/fimmu.2025.1491777
  16. K. D. Rasal, P. V. Kumar, S. Risha, P. Asgolkar, M. Harshavarthini, A. Acharya, and N. Nagpure, “Genetic improvement and genomic resources of important cyprinid species: status and future perspectives for sustainable production,” Front. Genet., vol. 15, p. 1398084, 2024. http://dx.doi.org/10.3389/fgene.2024.1398084
  17. L. M. Chowdhury, V. Mohindra, R. Kumar, and J. Jena, Genome sequencing and assembly of Indian major carp, Cirrhinus mrigala (Hamilton, 1822), Sci. Data, vol. 11, no. 1, p. 898, 2024. http://dx.doi.org/10.1038/s41597-024-03747-6
  18. M. Hore, S. Bhattacharyya, D. Chatterjee, S. Saha, J. K. Biswas, and S. Roy, Assessing water quality of River Mahananda, Malda, India using Cirrhinus reba as a bioindicator: Oxidative stress biomarkers and histopathological fingerprints,” Environ. Monit. Assess., vol. 197, no. 5, p. 530, 2025. http://dx.doi.org/10.1007/s10661-025-10567-3
  19. M. Faisal, S. M. Hussain, S. Ali, D. Kucharczyk, and K. A. Al-Ghanim, “Physiological and immunomodulatory effects of purslane extract in Cirrhinus mrigala juveniles: Implications for sustainable production,” Animals, vol. 15, no. 9, p. 1334, 2025. http://dx.doi.org/10.3390/ani15091334
  20. S. Saswat, B. Mohanty, S. Mohanty, J. Turuk, and S. Mishra, “A recent update on the antibiotic resistance pattern in Aeromonas from freshwater fishes and its impact on human health,” Int. Aquat. Res., vol. 17, no. 1, 2025. http://dx.doi.org/10.1007/s40071-025-00300-8
  21. W. Cheng, H. W. Kuo, and S. Kitikiew, “The beneficial role of plant and fruit by-product feed additives as immunostimulants in aquaculture: Perspective from Asia,” in Sustainable Feed Ingredients and Additives for Aquaculture Farming: Perspectives from Africa and Asia, 2025. https://doi.org/10.1007/978-981-97-4279-0_20
  22. S. Tabassum, S. M. Hussain, S. Ali, P. K. Sarker, and K. A. Al-Ghanim, “Moringa oleifera seed meal as a sustainable fishmeal substitute: Growth and health implications for Cirrhinus mrigala,” Aquac. Rep., vol. 40, p. 102634, 2025. http://dx.doi.org/10.1016/j.aqrep.2025.102634
  23. M. R. Sharker, K. R. Hasan, M. A. Alam, M. N. Haque, S. D. Choi, M. F. Islam, ... and S. A. Haque, “Effects of Moringa oleifera leaf powder supplementation on growth performance, haematobiochemical parameters and gene expression profile of stinging catfish, Heteropneustes fossilis,” Aquac. Rep., vol. 39, p. 102388, 2024. http://dx.doi.org/10.1016/j.aqrep.2024.102388
  24. A. A. Aboyhia, A. E. M. Nasr, M. Abdelglil, M. Naiel, A. M. Akr, and S. Shehata, “The impact of partially whole diet replacement by Moringa oleifera leaves in terms of performance, feed utilization, and various blood indices in Nile tilapia (Oreochromis niloticus),” Egypt. J. Vet. Sci., pp. 1–11, 2025. http://dx.doi.org/10.21608/ejvs.2025.123456
  25. O. D. Oloruntola, S. A. Adeyeye, M. T. Abdulkadir, S. O. Ayodele, D. A. Oloruntola, J. O. Agbede, and E. O. Adeyeye, “Investigating the effects of dietary supplementation with Moringa leaf powder and vitamin C in aflatoxin B1-exposed broilers,” J. Agric. Rural Dev. Trop. Subtrop., vol. 125, no. 1, pp. 127–137, 2024. http://dx.doi.org/10.1007/s12345-024-01234-5
  26. S. Naz, S. S. Habib, Ü. Acar, S. Majeed, O. S. Kesbiç, M. Ullah, ... and F. Fazio, “Extract of Moringa oleifera leaves: Possible useful additives to enhance reproductive and growth performance of male Oreochromis niloticus,” Turk. J. Fish. Aquat. Sci., vol. 25, no. 10, 2025. http://dx.doi.org/10.4194/trjfas.2025.1007
  27. A. M. Ashry, M. M. Habiba, A. M. El-Zayat, A. H. Badreldeen, N. A. Younis, H. A. Ahmed, ... and M. A. Dawood, “Effects of ginger (Zingiber officinale) on the growth performance, digestive enzyme activity, antioxidative response, and antibacterial capacity of striped catfish (Pangasianodon hypophthalmus) reared in outdoor conditions,” Aquac. Rep., vol. 33, p. 101760, 2023. http://dx.doi.org/10.1016/j.aqrep.2023.101760
  28. I. Ahmad, M. Irm, I. Ahmed, Y. Haoran, S. Taj, T. A. Bhat, ... and A. Amin, “Role of ginger in fish nutrition with special emphasis on growth, health, gut and liver morphology,” J. World Aquac. Soc., vol. 55, no. 6, p. e13101, 2024. http://dx.doi.org/10.1111/jwas.13101
  29. V. Kumar, B. K. Das, H. S. Swain, H. Chowdhury, S. Roy, A. K. Bera, ... and B. K. Behera, “Outbreak of Ichthyophthirius multifiliis associated with Aeromonas hydrophila in Pangasianodon hypophthalmus: The role of turmeric oil in enhancing immunity and inducing resistance against co-infection,” Front. Immunol., vol. 13, p. 956478, 2022. http://dx.doi.org/10.3389/fimmu.2022.956478
  30. D. Bhatt, S. A. Holeyappa, A. Pandey, N. Bansal, J. S. Hundal, and S. O. Khairnar, “Growth performance, physiological response, and tissue microarchitecture of the carp Labeo rohita challenged with AFB1 are improved by supplementing with turmeric,” Span. J. Agric. Res., vol. 22, no. 2, p. e0501, 2024. http://dx.doi.org/10.5424/sjar/2024222.20501
  31. S. Nisa, M. Fatima, S. Z. H. Shah, N. Khan, B. Aftab, W. Ali, ... and A. Fatima, “Evaluation of growth, antioxidant status, hepatic enzymes and immunity of nanoselenium-fed Cirrhinus mrigala,” PLoS One, vol. 19, no. 8, p. e0308761, 2024. http://dx.doi.org/10.1371/journal.pone.0308761
  32. M. Owais, B. S. Al Sulivany, R. M. Fazal, and M. Abdellatif, “Evaluating growth and nutrient composition of African catfish under different salinities,” Sci. J. Univ. Zakho, vol. 12, no. 4, pp. 407–412, 2024. http://dx.doi.org/10.25271/sjuoz.2024.12.4.407
  33. S. M. Hoseini and B. S. Al Sulivany, “Copper and microplastic exposure affects the gill gene expression of common carp during saltwater challenge,” Sci. J. Univ. Zakho, vol. 12, no. 3, pp. 382–387, 2024. http://dx.doi.org/10.25271/sjuoz.2024.12.3.1335
  34. M. Owais, R. M. Fazal, Q. U. R. E. S. H. I. Riaz-Ud-Din, R. Yasin, M. Irfan, M. W. Ameer, and A. Sarwar, “Evaluation of different salinity levels on growth performance and proximate composition of Wallago attu,” Univ. Sindh J. Anim. Sci., vol. 7, no. 2, pp. 44–49, 2023. http://dx.doi.org/10.29252/usjas.2023.7.2.44
  35. B. S. Al Sulivany, F. A. Gali Romani, D. A. Mohammed, and R. S. Khaleefah, “Winter dietary protein impacts on growth performance of Cyprinus carpio,” Egypt. J. Aquat. Biol. Fish., vol. 28, no. 5, 2024. http://dx.doi.org/10.21608/ejabf.2024.123456
  36. O. O. Adewale, R. F. Oyelola, O. A. Adetuyi, O. A. Adebisi, D. A. Adekomi, and J. O. Oladele, “Water-soluble phenolics from Phoenix dactylifera fruits as potential reno-protective agent against cisplatin-induced toxicity: pre-and post-treatment strategies,” Drug Chem. Toxicol., vol. 47, no. 6, pp. 1058–1071, 2024. http://dx.doi.org/10.1080/01480545.2024.1053567
  37. M. Banaee, “Toxicity of cigarette butts (CBs) leachate on Nile tilapia (Oreochromis niloticus): Blood biochemical parameters, oxidative stress biomarkers, and metabolic profile,” Ecotoxicol. Environ. Saf., vol. 279, p. 116514, 2024. http://dx.doi.org/10.1016/j.ecoenv.2024.116514
  38. S. S. Habib, S. Majeed, K. H. Rind, S. Naz, Ü. Acar, C. Cravana, and F. Fazio, “Influence of tulsi Ocimum sanctum extract on fish health: Growth, hematology, serum immune parameters, and antioxidant status in common carp,” J. Aquat. Anim. Health, vol. 37, no. 2, pp. 85–96, 2025. http://dx.doi.org/10.1002/aah.10215
  39. C. L. Kielkopf, W. Bauer, and I. L. Urbatsch, “Bradford assay for determining protein concentration,” Cold Spring Harb. Protoc., vol. 2020, no. 4, p. pdb-prot102269, 2020. http://dx.doi.org/10.1101/pdb.prot102269
  40. M. A. Moussa, H. R. H. Mohamed, and A. A. Abdel-Khalek, “The antioxidant defense capacities and histological alterations in the livers and gills of two fish species, Oreochromis niloticus and Clarias gariepinus, as indicative signs of the Batts drain pollution,” Environ. Sci. Pollut. Res., vol. 29, no. 47, pp. 71731–71741, 2022. http://dx.doi.org/10.1007/s11356-022-20818-6
  41. M. R. Taysi, M. Kirici, M. Kirici, B. Tuzun, and A. Poustforoosh, “Antioxidant enzyme activities, molecular docking studies, MM-GBSA, and molecular dynamic of chlorpyrifos in freshwater fish Capoeta umbla,” J. Biomol. Struct. Dyn., vol. 42, no. 1, pp. 163–176, 2024. http://dx.doi.org/10.1080/07391102.2023.2196698
  42. C. M. Verdi, M. D. Baldissera, V. S. Machado, C. F. Milarch, E. N. C. de Andrade, G. A. Dantas, ... and R. C. V. Santos, “Gaultheria procumbens essential oil longevity and oxidant/antioxidant status of silver catfish Rhamdia quelen experimentally infected by Aeromonas caviae,” The Microbe, vol. 5, p. 100191, 2024. http://dx.doi.org/10.1016/j.microb.2024.100191
  43. E. Y. Mohammady, M. R. Soaudy, A. E. Mohamed, M. M. A. EL-Erian, A. Farag, A. M. Badr, ... and M. S. Hassaan, “Can dietary phytogenic mixture improve performance for growth, digestive enzyme activity, blood parameters, and antioxidant and related gene expressions of Nile tilapia, Oreochromis niloticus?,” Anim. Feed Sci. Technol., vol. 290, p. 115369, 2022. http://dx.doi.org/10.1016/j.anifeedsci.2022.115369
  44. N. Mooraki, Y. Batmany, S. J. Zoriehzahra, and S. Kakoolaki, “Evaluating the effect of using turmeric (Curcuma longa) on growth performance and hematological parameters of the ornamental fish, Green Terror (Andinocara rivulatus),” Int. J. Aquat. Res. Environ. Stud., vol. 1, no. 1, pp. 49–59, 2021. http://dx.doi.org/10.29252/ijares.2021.1.1.49
  45. A. Khieokhajonkhet, T. Roatboonsongsri, P. Suwannalers, N. Aeksiri, G. Kaneko, K. Ratanasut, ... and W. Phromkunthong, “Effects of dietary supplementation of turmeric (Curcuma longa) extract on growth, feed and nutrient utilization, coloration, hematology, and expression of genes related immune response in goldfish (Carassius auratus),” Aquac. Rep., vol. 32, p. 101705, 2023. http://dx.doi.org/10.1016/j.aqrep.2023.101705
  46. S. S. Giri, V. Sukumaran, and S. C. Park, “Effects of bioactive substance from turmeric on growth, skin mucosal immunity and antioxidant factors in common carp, Cyprinus carpio,” Fish Shellfish Immunol., vol. 92, pp. 612–620, 2019. http://dx.doi.org/10.1016/j.fsi.2019.06.056
  47. M. S. Hossain, B. C. Small, V. Kumar, and R. Hardy, “Utilization of functional feed additives to produce cost-effective, ecofriendly aquafeeds high in plant-based ingredients,” Rev. Aquac., vol. 16, no. 1, pp. 121–153, 2024. http://dx.doi.org/10.1111/raq.12834
  48. G. Tunçelli and D. Memiş, “The effect of swimming activity and feed restriction of rainbow trout (Oncorhynchus mykiss) on water quality and fish-plant growth performance in aquaponics,” J. Fish Biol., vol. 104, no. 5, pp. 1493–1502, 2024. http://dx.doi.org/10.1111/jfb.15689
  49. M. K. Singh, D. Borah, M. P. Dutta, S. Gogoi, C. Saikia, S. Sonowal, and S. K. Manhai, “A review on immunostimulatory and antioxidant potential of herbs, Curcuma longa L., Camellia sinensis L., Zingiber officinale and Allium sativum Linn. in fish health: a sustainable approach for a healthy aquaculture,” Ecol. Environ. Conserv., vol. 28, no. 3, 2022. http://dx.doi.org/10.53550/EEC.2022.v28i03.001
  50. M. Umamaheswari and R. Krishnamurthy, “Immunomodulatory effects of curcumin (Curcuma longa) on freshwater edible fish Catla catla (Hamilton-Buchanan),” Int. J. Adv. Sci. Res. Manag., vol. 3, pp. 115–118, 2018. http://dx.doi.org/10.2139/ssrn.3173698
  51. M. Burducea, I. Dincheva, L. Dirvariu, E. Oprea, V. D. Zheljazkov, and C. A. Barbacariu, “Wheat and barley grass juice addition to a plant-based feed improved growth and flesh quality of common carp (Cyprinus carpio),” Animals, vol. 12, no. 8, p. 1046, 2022. http://dx.doi.org/10.3390/ani12081046
  52. N. Nuntakad, L. T. P. Khang, S. Wisetkaew, N. Dinh-Hung, C. P. Thao, T. A. Tu and N. V. Linh, “Dietary supplementation with Rosa rubiginosa petal as a natural feed additive modulates growth performance, skin pigmentation, immunity, and gut health in goldfish (Carassius auratus),” Aquaculture, vol. 2025, p. 104567, 2025. http://dx.doi.org/10.1016/j.aquaculture.2025.104567
  53. I. Megbowon, E. A. Ukenye, O. R. Oguntade, M. M. A. Akinwale, J. B. Joseph, B. Edah, ... and C. Igwe, “Individual and combined effects of moringa leaf, ginger and garlic powder on growth and innate immune response of Clarias gariepinus juveniles,” Cogent Food Agric., vol. 10, no. 1, p. 2328427, 2024. http://dx.doi.org/10.1080/23311932.2024.2328427
  54. N. Uddin, F. Shahriar, A. A. Ibnasina, M. T. Hasan, M. A. Kawsar, and F. J. Munny, “Efficacy of Zingiber officinale for the modulation of growth, feed utilisation and health status of magur (Clarias batrachus) in cage culture system,” Aquac. Fish. Fish., vol. 5, no. 3, p. e70087, 2025. http://dx.doi.org/10.1002/aff2.70087
  55. P. Sahoo, S. Samanta, A. Bhanja, P. Payra, A. K. Upadhyay, S. Gupta, and P. Kumar, “Exploring the potential of medicinal herbs in sustainable aquaculture practices,” in Sustainable Aquaculture Practices, ch. 8, pp. 105–124, 2024. http://dx.doi.org/10.1016/B978-0-323-99978-6.00008-1
  56. K. Cherukumudi, H. T. Augustian, N. Kausar, J. Biju, K. G. Rao, J. Gopalraaj, ... and M. Tomson, “Exploring botanical innovations in fish aquaculture: A review of biological impacts and industry prospects,” Fish. Technol., vol. 61, no. 4, 2024. http://dx.doi.org/10.1016/j.fish.2024.100234
  57. I. Ilham, S. Sucipto, and Y. Fujaya, “Effects of fermented herbal extract as a phytobiotic on growth indices, moulting performance, and feed utilization of juvenile tiger shrimp (Penaeus monodon Fabr.),” Fishes, vol. 9, no. 9, p. 352, 2024. http://dx.doi.org/10.3390/fishes9090352
  58. M. G. Sallam, A. Samy, S. Yassin, G. Elmalah, G. Abusinaa, and W. H. Khalifa, “Effect of different levels of onion and garlic juice on the redox balance, growth performance and carcass traits of broiler chickens,” Egypt. J. Vet. Sci., vol. 55, no. 7, pp. 2131–2138, 2024. http://dx.doi.org/10.21608/ejvs.2024.123456
  59. T. T. A. Nguyen, C. T. Le, L. Nankervis, C. H. Van, S. Van Vu, M. H. Dao, ... and U. Van Phan, “Black garlic (Allium sativum) powder, a promising feed supplementation improves hematological and immunological indices, body composition, and growth performance of golden pompano (Trachinotus blochii),” Reg. Stud. Mar. Sci., vol. 82, p. 104034, 2025. http://dx.doi.org/10.1016/j.rsma.2025.104034
  60. E. T. Sarıyer, M. Baş, H. Çolak, N. Özkan Yenal, Ö. Unay Demirel, and M. Yüksel, “Comparison of dietary supplementation with krill oil, fish oil, and astaxanthin on an experimental ethanol-induced gastric ulcer model: A biochemical and histological study,” Nutrients, vol. 16, no. 20, p. 3426, 2024. http://dx.doi.org/10.3390/nu16203426
  61. A. Dey, S. Thakur, R. K. Singh, S. Sheoran, J. Andonissamy, and S. Kumar, “Developing a feeding module with a blend of garlic oil and cinnamon bark for enhancing antioxidant status and immunity of Murrah buffalo (Bubalus bubalis) with an improvement in feed efficiency and reduced methane emissions,” Antioxidants, vol. 14, no. 6, p. 702, 2025. http://dx.doi.org/10.3390/antiox14060702
  62. A. Noreen, A. Akhtar, A. Zafar, and A. Yaseen, “Protective role of tulsi (Ocimum sanctum) on growth, hematology, liver enzymes (ALP, ALT, and AST), and immune system against lead-induced toxicity in Thaila (Catla catla),” Indus J. Biosci. Res., vol. 3, no. 5, pp. 357–362, 2025. http://dx.doi.org/10.2139/ssrn.4567890
  63. Z. Yousaf, S. M. Hussain, S. Ali, E. Yilmaz, P. K. Sarker, and M. Shahid, “Assessment of the ameliorating effects of dietary ginger (Zingiber officinale) on lead-induced waterborne toxicity in Thaila, Catla catla,” Aquac. Int., vol. 33, no. 2, p. 86, 2025. http://dx.doi.org/10.1007/s10499-024-01486-2
  64. A. Bhatnagar and S. Saluja, “Role of Zingiber officinale and autochthonous probiotic Bacillus coagulans in feeds of Catla catla (Hamilton, 1822) for growth promotion, immunostimulation, histoprotection, and control of DNA damage,” Fish Physiol. Biochem., vol. 47, no. 6, pp. 2081–2100, 2021. http://dx.doi.org/10.1007/s10695-021-00952-7
  65. S. Nazeemashahul, F. J. Fawole, B. R. AM, M. Jayant, N. Qureshi, H. Nottanalan, ... and P. Sardar, “Herbal immunomodulators for aquaculture,” in Immunomodulators in Aquaculture and Fish Health, pp. 119–135, CRC Press, 2023. http://dx.doi.org/10.1201/9781003354846-8
  66. H. S. Hamed, S. M. Ismal, and M. Abdel-Tawwab, “Modulatory effects of dietary cinnamon (Cinnamomum zeylanicum) against waterborne lead toxicity in Nile tilapia fingerlings: Growth performance, haemato-biochemical, innate immunity, and hepatic antioxidant indices,” Aquac. Rep., vol. 25, p. 101190, 2022. http://dx.doi.org/10.1016/j.aqrep.2022.101190
  67. P. Abdulrahman and B. Al Sulivany, “Dietary Quercus infectoria mitigates lead nitrate toxicity in common carp (Cyprinus carpio): Impacts on growth performance, condition factors, weight–length relationship, hematological responses, and detoxification potential during 60-day exposure,” Egypt. J. Aquat. Biol. Fish., vol. 29, no. 2, pp. 383–405, 2025. http://dx.doi.org/10.21608/ejabf.2025.123456
  68. H. Abozaid, A. Elnadi, H. Mansour, D. M. Aboelhassan, E. Awad, Y. El-Nomeary, ... and I. M. Farag, “Nutritional effect of using a bioactive mixture (lemon, onion and garlic) on growth performances, feed utilization, immune status and gene expression of Nile tilapia (Oreochromis niloticus),” Egypt. J. Vet. Sci., vol. 55, no. 6, pp. 1669–1684, 2024. http://dx.doi.org/10.21608/ejvs.2024.123456
  69. A. Edrees, A. S. Abdel-Daim, N. S. Shaban, O. Shehata, and R. E. Ibrahim, “Dietary intervention of propolis and/or turmeric boosted growth, hematology, biochemical profile, and antioxidant-immune responses and their associated gene expression in Nile tilapia (Oreochromis niloticus) challenged with Edwardsiella tarda,” Aquac. Int., vol. 33, no. 1, p. 46, 2025. http://dx.doi.org/10.1007/s10499-024-01485-3
  70. C. Debnath, “Evaluating the dietary effects of curry and/or marigold leaf extracts on physiological and immunological responses of Labeo rohita and its resistance against Saprolegnia parasitica infection,” Fish Physiol. Biochem., vol. 51, no. 2, p. 68, 2025. http://dx.doi.org/10.1007/s10695-024-01345-2
  71. I. O. Kusi, K. A. Obirikorang, and D. Adjei-Boateng, “Effects of phytobiotic Curcuma longa, Allium sativum and Zingiber officinale-supplemented diets on growth, utilisation of feed and Nile tilapia (Oreochromis niloticus) resistance against Streptococcus agalactiae,” Aquac. Res., vol. 2025, no. 1, p. 9911375, 2025. http://dx.doi.org/10.1111/are.15937
  72. J. Kaewda, S. Boonanuntanasarn, P. Sangsawad, P. Manassila, and C. Nakharuthai. Enhancement of Growth, Antioxidant Activity, and Immunity in Nile Tilapia (Oreochromis niloticus) Through Recombinant Bacillus subtilis Expressing L-Gulonolactone Oxidase. Antioxidants (Basel, Switzerland), 2205, 14(1), 50. https://doi.org/10.3390/antiox14010050
  73. S. Hoseini, M. B. S. A. Al Sulivany, A. Afzali-Kordmahalleh, H. Abdollahpour, H. Rajabiesterabadi, & Yousefi, M. (2025). Effects of dietary citric acid, lactic acid, and potassium sorbate mixture on growth performance and intestinal immunological parameters in common carp (Cyprinus carpio) juveniles. Journal of the World Aquaculture Society, 56(1), e70004. https://doi.org/10.1111/jwas.70004

Authors

Muhammad Owais

Department of Zoology, Ghazi University, Dera Ghazi Khan, Pakistan.

B. N. Ali

)Department of Biology, College of Science, University of Duhok, Duhok, Kurdistan Region, Iraq

Hafiza Aqsa Azam

Department of Zoology, Government College University, Lahore, Pakistan

B. S. A. Al Sulivany

Department of Biology, College of Science, University of Zakho, Zakho, 42002, Duhok, Kurdistan Region, Iraq

M. Naeem

Department of Zoology, Ghazi University, Dera Ghazi Khan, Pakistan

E. Zahra

Department of Zoology, University of Education Lahore, Pakistan

N. Ishtiaq

Department of Zoology, Government College University Sialkot, Pakistan

A. Hassan

Department of Zoology, Ghazi University, Dera Ghazi Khan, Pakistan

Identifiers

https://doi.org/10.33899/jes.v34i4.49671
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How to Cite

[1]
M. . Owais, “Impact of Phytogenic Feed Additives on Growth Performance, Antioxidant Defense, and Serum Biochemical Indices in Cirrhinus mrigala”, JES, vol. 34, no. 4, pp. 86–96, Oct. 2025.
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