Qualitative and Quantitative Determination of Salicylic acid and Resorcinol in Callus of Calendula officinalis L. Using HPLC Technique

Parts of leave of Calendula officinalis plant before and after blooming were cultured on MS medium supplemented with 30 g / l scores, 6 g / l agar, pH was 5.7 0.1, in addition to 2,4-D at (0.0 , 0.1 , 0.2 , 0.5) mg/ l for callus induction, produced callus recultured on medium supplemented with ( 0.0, 0.5, 1.0, 1.5, 2.0) mg/ l NAA interaction with 0.1 mg/ l BA for three times three weeks for each one. Salicylic acid and Resorcinol were stimulated and identified in callus using High Performance Liquid Chromatography (HPLC) method. The data indicate: highest fresh weight of callus 13.04 gm achieved from cultured parts of leaves before flowering on medium supplemented with 1.0 mg/ l NAA and 0.1 mg/ l BA after nine weeks, and the highest fresh weight of callus 20.50 g obtained from cultured parts of leaves during flowering on medium supplemented with 1.5 mg/ l NAA and 0.1 mg/ l BA after nine weeks, highest quantity of salicylic acid 30.02 mg/gm dry weight was obtained from callus produced from parts of leaves before flowering cultured on MS medium supplemented with 1.5 mg/ l NAA and 0.1 mg/ l BA after six weeks, highest quantity of Resorcinol 139.28 mg/ gm dry weight was obtained from callus produced from parts of leaves took during flowering cultured on MS medium supplemented with 2.0 mg/l NAA and 0.1mg/ l BA after six weeks.