Partial Purification and Study of Osteocalcin from Blood of Human and Rats

This study is concerned with attempts to isolate and purify osteocalcin from healthy human blood plasma and healthy rats blood serum using different biochemical techniques. This included precipitation by cold acetone and gel filtration chromatography. It was found that only the second peak (peak B) from human and rats had concentration of osteocalcin, and showed that fold of purification of isolated osteocalcin 57 and 43 for human and rats respectively. The results obtained from electrophoresis showed that one single band for isolated osteocalcin (peak B) from human and rats, and from high performance liquid chromatography showed that there was a good identical in retention time between the standard and isolated osteocalcin (peak B) from human and rats. The comparative molecular weight of partially purified osteocalcin from human plasma and rats serum was (6011.73 400 Da) and (5248.07 400 Da) respectively using gel filtration chromatography, and (5807.64 Da) and (4786.30 Da) respectively using electrophoresis technique. .